Journal: Reproductive biology and endocrinology : RB&E

Article Title: Betaine attenuate chronic restraint stress-induced changes in testicular damage and oxidative stress in male mice.

doi: 10.1186/s12958-022-00949-8

Figure Lengend Snippet: Fig. 5 Biochemical analysis of antioxidant status. Each bar represents the mean ± SEM (N = 6 male mice). (A) Effect of BET on the levels of oxidized glutathione (GSSG) in the testis of CRS mice; (B) BET on the levels of CRS mice The effect of reduced glutathione (GSH) level in testis tissue; (C) The effect of BET on the content of malondialdehyde (MDA) in testis tissue of CRS mice; *P < 0.05, compared with Control group; ***P < 0.001, compared with Control group; ###P < 0.001, compared with CRS + NS group; Different symbol indicate significant difference P < 0.05 among groups determined by one-way ANOVA (followed by Bonferroni post hoc test)

Article Snippet: The homogenates were filtered and centrifuged at 3500 rpm and 4 °C for 15 min using a refrigerated centrifuge and the activities of MDA (Solarbio, BC0025, China), GSSG (Elabscience, E-BC-K097-M, China) and GSH (Elabscience, E-BC-K030-M, China) were determined according to the instruction.

Techniques: Control

Journal: The Journal of biological chemistry

Article Title: Cytokine-mediated induction of ceramide production is redox-sensitive. Implications to proinflammatory cytokine-mediated apoptosis in demyelinating diseases.

doi: 10.1074/jbc.273.32.20354

Figure Lengend Snippet: FIG. 4. Depletion of intracellular GSH concentration by TNF-a and IL-1b in rat primary astrocytes is restored by NAC. Cells preincubated with 10 mM NAC for 1 h received either TNF-a (50 ng/ml) or IL-1b (50 ng/ml). At different time intervals, cells were scraped off, and GSH concentrations (100% value is 182.5 6 15.4 nmol/mg protein) were measured as described under “Materials and Methods.” Results are mean 6 S.D. of three different experiments.

Article Snippet: Measurement of GSH (Reduced Glutathione) and GSSG (Oxidized Glutathione)—Concentration of intracellular reduced GSH was measured using a colorimetric assay kit for GSH from R & D Systems.

Techniques: Concentration Assay

Journal: The Journal of biological chemistry

Article Title: Cytokine-mediated induction of ceramide production is redox-sensitive. Implications to proinflammatory cytokine-mediated apoptosis in demyelinating diseases.

doi: 10.1074/jbc.273.32.20354

Figure Lengend Snippet: FIG. 5. Effect of diamide, a thiol-depleting agent, on the induc- tion of ceramide production in rat primary astrocytes. Cells preincubated with 10 mM NAC for 1 h received diamide (0.5 mM). At different time intervals, cells were washed with HBSS and scraped off. A, lipids were extracted, and the level of ceramide (100% value is 4.51 6 0.1 nmol/mg protein) was measured as described under “Materials and Methods.” Results are mean 6 S.D. of three different experiments. B, at different time intervals, intracellular level of GSH (100% value is 182.5 6 15.4 nmol/mg protein) was measured as described under “Ma- terials and Methods.” Results are mean 6 S.D. of three different experiments.

Article Snippet: Measurement of GSH (Reduced Glutathione) and GSSG (Oxidized Glutathione)—Concentration of intracellular reduced GSH was measured using a colorimetric assay kit for GSH from R & D Systems.

Techniques: